Clinicopathologic significance of LAIR-1 expression in hepatocellular carcinoma

Aim

Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is an immune inhibitory receptor which is expressed within most types of hematopoietic cells and negatively regulates immune responses. Recently, we found LAIR-1 expression to be present within tumors of nonhematopoietic lineages. However, the roles of LAIR-1 in hepatocellular carcinoma (HCC) have yet to be examined. The purpose of this study was to investigate the expression of LAIR-1 in HCC tissue and assess its clinical significance at this site.

阿的平诱导结外NK/T淋巴瘤细胞凋亡的实验研究

背景与目的：随着含有门冬酰胺酶化疗方案以及局部放疗和免疫检查点抑制剂的成功应用，结外NK/T细胞淋巴瘤［extranodal natural killer（NK）/T-cell lymphoma，ENKTCL］患者缓解率和无病生存率较以往有显著提高，然而仍有相当一部分进展期ENKTCL患者成为复发/难治性病例。前期研究发现，抗寄生虫小分子化合物阿的平可以抑制包括血液肿瘤在内多种肿瘤细胞增殖并诱导其凋亡，可能成为新型抗肿瘤药物。进一步探究阿的平对ENKTCL细胞的影响及其可能作用机制。方法：以不同浓度阿的平处理ENKTCL细胞系SNK6和NK92（分别由上海交通大学医学院附属新华医院血液内科和上海血液学研究所提供），观察细胞生长和形态并采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）检测其增殖，同时应用流式细胞术检测细胞周期、凋亡率以及细胞内线粒体跨膜电位和活性氧水平，进一步利用蛋白［质］印迹法（Western blot）检测细胞自噬相关蛋白LC3B和P70表达的变化。结果：SNK6和NK92细胞经阿的平处理48 h后，细胞增殖抑制率分别为（47.08±2.19）%和（30.46±7.95）%，明显高于对照组 ［（11.85±1.89）%和（10.08±2.01）%，P值均<0.05］，阿的平处理24 h后处理组SNK6和NK92细胞凋亡率分别达到（86.45±6.54）%和（76.5±10.8）%；显著高于对照组 ［（3.3±3.24）%和（2.64±1.67）%，P值均<0.05］。同时阿的平处理组SNK6和NK92细胞周期均发生明显S期阻滞，但细胞内线粒体跨膜电位均无显著变化。进一步研究还发现，阿的平能够促使SNK6细胞内活性氧水平升高并抑制SNK6和NK92细胞内P70蛋白磷酸化，同时上调LC3B蛋白表达。结论：阿的平能够诱导ENKTCL细胞增殖阻滞和凋亡，其机制可能与阿的平介导细胞内活性氧水平升高以及抑制mTOR信号通路进而激活细胞自噬有关。     

Homocysteine might increase the risk of recurrence in patients presenting with primary cerebral infarction

Purpose: Although hyperhomocysteinemia (Hhcy) is a risk factor for cerebral infarction, its effect on recurrent cerebral infarction is less-defined. We aimed to investigate the association of Hhcy and increased risk of recurrent cerebral infarct.

Materials and methods: From 2011 to 2013, we recruited 231 primary cerebral infarct patients that were divided to a Hhcy group (n?=?105) and a control group (n?=?126) according to plasma homocysteinemia (Hcy) levels exceeding 15?μmol/L. In this prospective study, risk factors such as gender, age, blood lipid and glucose levels, history of diabetes, high blood pressure, smoking habits and plasma Hhcy levels were determined. A three-year follow-up compared differences in cerebral infarction recurrence rates. Statistical analyses identified whether plasma Hhcy levels were an independent risk factor for recurrent cerebral infarction.

Results: Triglyceride and low-density lipoprotein (LDL) levels in the Hhcy group were significantly higher than controls, and cerebral infarct recurrence rates in the Hhcy group exceeded control subject rates through the three-year follow-up (p?=?.021, p?=?.036 and p?=?.025). Cox proportional hazards modeling showed that elevated Hhcy levels (hazard ratio [HR]?=?3.062, p?<?.001), increased age (HR?=?1.069, p?<?.01), circulating triglyceride levels (HR?=?1.686, p?=?.048), and relative National Institutes of Health Stroke (NIHSS) score (HR?=?1.068, p?=?.016) were risk factors for recurrent cerebral infarction.

Conclusions: Level of Hhcy was a risk factor for recurrent cerebral infarction. Further, particular demographic and clinical outcomes including age, relative NIHSS scores, and circulating triglyceride levels were markedly associated with the occurrence of cerebral infarction.     

Characteristics and outcomes of in-hospital cardiac arrest in adults hospitalized with acute coronary syndrome in China

AimsThis retrospective study aims to analyze and explore the clinical characteristics, risk factors, and in-hospital outcomes - including return of spontaneous circulation (ROSC) and survival to discharge - of hospitalized patients admitted with acute coronary syndrome (ACS) suffering cardiac arrest.MethodsACS patients admitted to three tertiary hospitals in Fujian, China, were evaluated retrospectively from January 1, 2012 to December 30, 2016. Data were collected, based on the Utstein Style, for all cases of attempted resuscitation for IHCA. We analyzed patient characteristics, pre-event variables, event variables, and the main outcomes, including ROSC and survival to discharge, and identified the influencing factors on the outcomes.ResultsThe total number of ACS admissions across the three hospitals during this study period was 21,337. Among these admissions, 320 ACS patients experienced IHCA (incidence: 1.50%); 134 (41.9%) patients experienced ROSC; and 68 (21.2%) survived to discharge. The findings indicated that four factors were associated with ROSC, including age <70 years-old, shockable rhythm, duration of resuscitation (≤15 min and 16–30 min), and PCI. Five factors were associated with survival to discharge, including age <70 years-old, shockable rhythm, the duration of resuscitation (≤15 min and 16–30 min), Killip ≤ II, and CCI ≤ 2.ConclusionYounger age, shockable rhythm, and shorter duration of resuscitation were all factors demonstrated to be a predictor of ROSC and survival to hospital discharge.     

Treatment of skeletal open bite using a navigation system: CAD/CAM osteotomy and drilling guides combined with pre-bent titanium plates

Severe skeletal open bite associated with posterior vertical maxillary excess and mandibular deformity is considered a difficult problem in orthodontic and surgical treatment. This study used a navigation system for the correction of severe skeletal open bite in order to accurately transfer the virtual plan to the actual operation and achieve precise rigid internal fixation in bimaxillary osteotomies of the jaws. Twelve patients with a severe skeletal open bite associated with vertical maxillary excess and mandibular deformity were recruited. All patients underwent Le Fort I osteotomy and bilateral sagittal split ramus osteotomy with the guidance of this navigation system. Computed tomography and cephalometric examinations were performed to evaluate the correction of the deformity. Deviations between the simulated plan and actual postoperative outcome were measured to determine the precision of the surgery. Satisfactory and stable results were achieved in all patients postoperatively, without complications or relapse during follow-up. Photographs and cephalometric evaluations showed that the facial profile and occlusion were improved. Assessment of the deviations between the simulated plan and actual postoperative outcome showed that the navigation system can precisely transfer the virtual plan to the actual operation. The results suggest that the navigation system can accurately transfer the virtual plan to the actual operation during bimaxillary jaw osteotomies, without relapse, in patients with a severe skeletal open bite.     

Double‐chambered left ventricle diagnosis by 2D and 3D echocardiography: From fetus to birth

Double‐chambered left ventricle (DCLV) is a rare cardiac anomaly and is characterized by the division of the left ventricle by abnormal muscle and/or fiber bundles into two chambers. We hereby report a fetus which was diagnosed with DCLV in utero by 2D and 3D Fetal echocardiography and the findings were confirmed after birth.     

Genomic and Transcriptomic Landscape of Triple-Negative Breast Cancers: Subtypes and Treatment Strategies

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